ACQUISITION REQUEST FOR AN INSTRUMENT FOR THE LABORATORY OF THE STEM CELL TRANSPLANT AND CELLULAR THERAPY CENTRE
Studies are in progress in the Stem Cell Transplant Laboratory of the Regina Margherita Hospital of Turin regarding the mesenchymal stem cells (MSC). The MSC are drawn from a healthy individual and cultivated in a laboratory to be able to transplant them in diseased subjects for therapeutic purposes. In this case, the human MSC are isolated and expanded, originating from the medulla ossium (bone marrow) and driven to differentiation in a neutral sense in vitro.
The differentiation of the MSC into neutral cells is studied by means of biological cellular, molecular and electrophysiological techniques. In particular, the MSC placed in culture with diverse agents of differentiation including ß-mercaptoethanol; dimethyl sulfoxide + ß-hydroxyanisole; trans-retinoic acid and Neural Progenitor Maintenance Medium are able to modify their morphology (assuming a semi-neural aspect), express neural markers (including Nestin, Neuron-Specific Enolase; the antigen Neuronal Nuclear, the type 2 Microtubule-associated Protein and the Glial Fribrillary Acidic Protein) and present currents associated to the K+ channels raised by means of electrophysiological techniques. These cells (MSC) are able to excrete substances which favour the growth of the neurons and their prolongation.
Moreover, to study the capacity of the MSC to slow the progression of the neuronal damage and the eventual regeneration of the damaged nerve tissue, in the Human Anatomy and Legal Medicine laboratory of the University of Studies in Turin, directed by Prof. Alessandro Vercelli, in vivo studies are in progress on an experimental model of amyotrophic lateral sclerosis in mice. As a model, SOD-1 mice are used, that is a carrier of the mutated gene of the superoxide-dimutase of type 1. Such mutation provokes in mice a clinical picture similar to that of the patients afflicted with the familial form of Amyotrophic Lateral Sclerosis, a disease characterised by the progressive degeneration of motoneurons with consequent atrophy and muscular paralysis. The human MSC are marked with a nuclear colorant (Bisbenzimide) and inoculated in the spinal cord level. The results obtained to date demonstrate how the MSC inoculated at a spinal cord level of these mice are able to root, migrate, and survive in the long term and reduce the reactive astrogliosis, slowing the progression of the pathology. Such results reflect from a functional point of view in the improvement in the behavioural tests, therefore, in the motor functions in the transplanted mice. It is our intention to use the MSC to administer substances that prevent the neuronal death in the spinal cord location.
To continue such studies, the use of new instruments are deemed necessary including a laminar flow hood for the manipulation of cellular cultures with UV lamp and empty tap and a large capacity centrifuge counter ventilated for the isolation and expansion of the mesenchymal stem cells.
Dott.ssa Franca Fagioli
STEM CELL TRANSPLANT AND CELLULAR THERAPY CENTRE
University of Studies of Turin
Regina Margherita Hospital
Piazza Polonia, 94 – Turin
24/08/06 – – Thermo Electron Corporation’s confirmation order:
Price reserved for us 11,266.00 Euro
code 82024100: Maxi Safe 2010 1.8/ with divided work station
code 52270240: UV Lamp with timer
Price reserved for us 4,785.00 Euro
code 11177560: Centrifuge C4i
code 11175338: M4 oscillating rotor
code 11174218: Set 4 vial racks
code 11174529: Set 4 adaptors
Price reserved for us 5,258.40 Euro
code 19371: Incubator + 8 door Kit
Total order in Euro 21,309.40 + vat 20%= Euro 25,571.28. (Fatt. n. d/2 06 00 2508 del 27/12/2006 Thermo Electron Corporation)
10/01/2007: Downpayment for equipment ((invoice n. d/2 06 00 2508 Thermo Fisher Scientific) € 6.392,82
15/07/2008: Paid balance of € 19.178,46 for equipment (invoice. n. d/2 06 00 2508 Thermo Fisher Scientific)